GENOGATE [food and environmental tests utilizing DNA chip technology]ジェノゲートHOME

  • What is DNA chip testing?
  • Basic principles
  • Outline of tests for food poisoning bacteria
  • Outline of fungi tests
  • Detection machine
  • Download reference materials

“Being a substrate on which a number of biomolecules (DNA, proteins, sugar chains) are fixed, a biochip is a device that allows you to detect target molecules or compounds that have specific interactions with the biomolecules on the chip in a massive and simultaneous manner. Among the biochips, that on which DNA is fixed is called a DNA chip (DNA microarray)” (source: Japan Multiplex bio-Analysis Consortium website).

GENOGATE chip (DNA chip)

GENOGATE chip is a DNA chip on which a number of short, single-stranded DNA fragments (probes) that detect specific DNA sequences in the sample, are fixed. Taking advantage of the behavior whereby a nucleotide forms a pair (double-strand) with complemental nucleotide, the probe on the chip substrate detects the target DNA sequence that exists in the sample in a simultaneous manner.
By applying DLC (Diamond-Like Carbon) coating to the surface of the silicon substrate, we achieved low-noise, high-quality signals of the highest level of the class, allowing stable and highly sensitive detection.

  • Since chemical modification is possible, DNAs will be tightly bound.
  • Carbon density is high and the amount of immobilization density of the DNA is high.
  • The densely arranged spots on the substrate detect multiple samples simultaneously.

GENOGATE
GENOGATE

GENOGATE test flow (in case of fungi tests)

1 Culture the test sample so that all the fungi in the sample multiply.
2 Extract DNA from the multiplied fungi.
3 Apply the extracted DNA to PCR to amplify the target DNA. At this stage, a fluorescent marker is attached when the target DNA is amplified.
4 Hybridization with the DNA chip is carried out.
5 Wash the DNA chip to remove unbound DNA.
6 Set the DNA chip in the detection machine. Laser beam is applied to excite the energy of the fluorescent marker attached to the target DNA.
7 Fluorescence emitted by the fluorescent marker is scanned by a cooled CCD camera installed in the detection machine resulting in a low-noise image.
8 The scanned DNA chip image is analyzed to work out the fluorescence intensity of each spot.
9 S/N ratio is worked out from the fluorescence intensity to judge if the target DNAs are present.

Further enquiry

Gene Testing Group, Life Science Business Promotion Office, Tel: 03-4514-2003Enquiry Form


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