An Innovative Spheroid Culture Tool
Large size WELLBAG
WELLBAG-L350

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“WELLBAG-L350”Outline

Large size WELLBAG

This is a scale-up product from the WELLBAG-S series products. It has 20 times culture area of WELLBAG-S series. The number of wells is about 36 times that of S500 and about 20 times that of S350. The bag is A3 size and it can be placed in a standard type incubator with a dedicated holder.

Movie

  • WELLBAG-L350 Operation Overview Video

Five Features

1. WELLBAG is a closed bag system which reduces the contamination risk of bacteria and foreign substances as opposed to “Open system containers”.

No contamination risk.
There is a risk of contamination with foreign substances and/or bacteria when the lid is opened.

2. Mass culture of uniformly sized spheroids

Fine and uniform microwells are processed on the bottom surface of WELLBAG with non-cell-adhesive coating, while top surface of WELLBAG is has an overhang part. When the cell suspension is injected into the bag from the injection port, the cell suspension is distributed almost evenly throughout the bag and by leaving it still, each microwell contains approximately the same number of cells. As a result, uniform size of spheroid is formed in each microwell.

WELLBAG-L350
Size 292mm × 412mm
Material Polyethylene with non-cell adhesion coating
Culture Area 1,000cm2
Culture volume 400mL
Well diameter 350μm
Number of wells ca. 650, 000
Spheroids of uniform size
  • *Measurement using "Multisizer 4e" by Beckman Coulter K.K.

3. Efficient gas exchange due to high gas permeability film

A high gas permeability film is used to ensure sufficient gas exchange of medium in a closed culture system.

4. Safety standards according to the Japanese Pharmacopoeia

Cytotoxicity test Confirmed
Leachable test Confirmed
Ashing test Confirmed
Endotoxin test Less than 0.25EU/mL
Sterility Radiation Sterilization
  • Ref.) Japanese Phamacopoeia, 18th rev., 2021

5. It can be handled using a large bag dedicated holder.

Although the size of the bag is fit to a standard incubator, please check the size and the load capacity of the shelf inside the incubator before use.

Holder for WELLBAG-L350
Size W:423mm、D:294mm、H:41mm
Materials Aluminum, Tempered Glass, Stainless steel, Polycarbonate, others
Compatible bag WELLBAG-L350
Weight 3.8kg
  • Note: Regarding application to insulin-producing cells, including pancreatic progenitor cells, there may be restrictions depending on the intended use. So, please contact us in advance if you intend to use WELLBAG to these applications.

FAQ

  • Q.1Where can I buy a WELLBAG-L350?
    A.1

    This is a made-to-order product. Please contact our local distributor or Toyo Seikan Group Holdings, Ltd.

  • Q.2Can I use the WELLBAG-L350 without the dedicated holder?
    A.2

    Because of the WELLBAG is a soft type culture bag, it is necessary to use it together with the dedicated holder to prevent the movement of spheroids among microwells.

  • Q.3What is the body of the WELLBAG-L350 made of?
    A.3

    Polyethylene of high gas permeability grade.

  • Q.4Can the WELLBAG-L350 be used repeatedly?
    A.4

    It is recommended for one-time use.

  • Q.5Can the WELLBAG-L350 be used for medium exchange?
    A.5

    No, it cannot. The bag is designed only to form and collect spheroids.

  • Q.6What is the well diameter and well quantity for the WELLBAG-L350?
    A.6

    The WELLBAG-L350 has a diameter of 350 µm and a quantity of 650,000 wells.

Contact

Toyo Seikan Group Holdings, Ltd.
Business Management Group, Business Innovation Office
2-18-1, Higashi-Gotanda, Shinagawa-ku, Tokyo 141-8627, Japan
E-mail: bis_innov_wellbag@tskg-hd.com