An Innovative Spheroid Culture Tool
WELLBAG
WELLBAG-S500

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“WELLBAG-S500”Outline

A new spheroid formation tool that achieves “closed system”, “uniform size in large quantities”, and “easy collection”.

WELLBAG is a culture bag specially designed to culture spheroids uniformly in large quantities and to recover formed spheroids quite easily for various cells including iPS cells, which are used in regenerative medicine etc. This product is used by attaching to a dedicated holder.
WELLBAG-S500 has 18,000 microwells with a well diameter of 500um.

Movie

  • Innovative Spheroid Culture Bag "WELLBAG"

  • WELLBAG-S500 Operation Overview Video

References

Seven Features

1. WELLBAG is a closed bag system which reduces the contamination risk of bacteria and foreign substances as opposed to “Open system containers”.

No contamination risk.
There is a risk of contamination with foreign substances and/or bacteria when the lid is opened.

2. Mass culture of uniformly sized spheroids

Fine and uniform microwells are processed on the bottom surface of WELLBAG with non-cell-adhesive coating, while top surface of WELLBAG is has an overhang part. When the cell suspension is injected into the bag from the injection port, the cell suspension is distributed almost evenly throughout the bag and by leaving it still, each microwell contains approximately the same number of cells. As a result, uniform size of spheroid is formed in each microwell.

WELLBAG-S500
Size 72mm × 120mm
Material Polyethylene with non-cell adhesion coating
Culture volume 10 – 20mL
Well diameter 500 μm
Number of wells ca. 18,000
Timelapse of spheroid formation

3. Easy collection of spheroids after the culture and no damage to spheroids during collection

Turn the bag upside down to drop the formed spheroids, and then collect the spheroids with a syringe. Compared to conventional culture methods using petri dished, flasks, etc., it is much easier and can be recovered without damaging spheroids.

4. Efficient gas exchange due to high gas permeability film

A high gas permeability film is used to ensure sufficient gas exchange of medium in a closed culture system.

5. High operability using a dedicated holder

By attaching the WELLBAG to a dedicated holder, the movement of spheroids between wells is suppressed and portability becomes possible. Parts of the base plate and holding plate are transparent, allowing microscopic observation while attached.

Holder
Size W: 128mm, D: 86mm, H: 20mm
Materials Aluminum, Glass, Stainless steel, Polycarbonate, Neodymiun magnet
Compatible bag WELLBAG-S500, WELLBAG-S350
Weight 300g

6. Safety standards according to the Japanese Pharmacopoeia

Cytotoxicity test Confirmed
Leachable test Confirmed
Ashing test Confirmed
Endotoxin test Less than 0.25EU/mL
Sterility Radiation Sterilization
  • Ref.) Japanese Phamacopoeia, 18th rev., 2021

7. Easy to remove air bubbles inside the wells.

There are two ways to remove air bubbles, Manual method and Incubation method.

  • Please refer the operation manual of WELLBAG for details.
  • Note: Regarding application to insulin-producing cells, including pancreatic progenitor cells, there may be restrictions depending on the intended use. So, please contact us in advance if you intend to use WELLBAG to these applications.

Item

Product No. Product name Number of items
11000
-05
WELLBAG-S500 5
11000
-10
WELLBAG-S500 10
11000
-00
WELLBAG-S500 100
61000
-01
WELLBAG HOLDER 1
  • Please contact the following distributors for pricing.

FAQ

  • Q.1Where can I buy a WELLBAG-S500?
    A.1

    It is available by our local distributors. See distributors list.

  • Q.2Can I use the WELLBAG-S500 without the special holder?
    A.2

    Because of the WELLBAG is soft type culture bag, it is necessary to use it together with the dedicated holder to prevent the movement of spheroids among microwells.

  • Q.3What material is used for the body of WELLBAG?
    A.3

    Polyethylene of high gas permeability grade.

  • Q.4Can the WELLBAG-S500 be used repeatedly?
    A.4

    It is recommended for one-time use.

  • Q.5Can the WELLBAG-S500 be used for medium exchange?
    A.5

    No, it can’t. WELLBAG-S500 is designed to culture spheroids without medium exchange.

  • Q.6What is the well diameter of the WELLBAG-S500?
    A.6

    500㎛

  • Q.7What kind of cell spheroids can I make with the WELLBAG-S500?
    A.7

    It depends on the cell type used and culture conditions. We have many experiences using iPS cells and MSCs.

  • Q.8What is the expiration period of the WELLBAG-S500?
    A.8

    Three years after manufacture.

Contact

Toyo Seikan Group Holdings, Ltd.
Business Management Group, Business Innovation Office
2-18-1, Higashi-Gotanda, Shinagawa-ku, Tokyo 141-8627, Japan
E-mail: bis_innov_wellbag@tskg-hd.com

Distributor in USA