Overview
This is a scale-up product from the WELLBAG-S series products. It has 20 times culture area of WELLBAG-S series. The number of wells is about 36 times that of S500 and about 20 times that of S350. The bag is A3 size and it can be placed in a standard type incubator with a dedicated holder.
Lineup
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WELLBAG-L350
Features
1. WELLBAG is a closed bag system which reduces the contamination risk of bacteria and foreign substances as opposed to “Open system containers”.
WELLBAG is a closed bag system which reduces the risk of contamination compared with open systems such as dishes and flasks.
2. Mass culture of uniformly sized spheroids
Fine, uniform microwells are formed on the bottom surface of WELLBAG with a non-cell-adhesive coating, while the top surface of WELLBAG has an overhang structure. When a cell suspension is injected into the bag through the injection port, it spreads almost evenly throughout the bag. By leaving it undisturbed, each microwell contains approximately the same number of cells. As a result, spheroids of uniform size are formed in each microwell.
| Size | 292mm × 412mm |
|---|---|
| Material | Polyethylene with non-cell adhesion coating |
| Culture area | 1,000cm2 |
| Culture volume | 400mL |
| Well diameter | 350μm |
| Number of wells | ca. 650,000 |
Spheroids of uniform size
*Measurement using "Multisizer 4e" by Beckman Coulter K.K.
3. Efficient gas exchange due to high gas permeability film
A high gas permeability film is used to ensure sufficient gas exchange of medium in a closed culture system.
4. Safety standards according to the Japanese Pharmacopoeia
| Cytotoxicity test | Confirmed |
|---|---|
| Leachable test | Confirmed |
| Ashing test | Confirmed |
| Endotoxin test | Less than 0.25EU/mL |
| Sterility | Radiation Sterilization |
5. It can be handled using a large bag dedicated holder.
Although the size of the bag is fit to a standard incubator, please check the size and the load capacity of the shelf inside the incubator before use.
| Size | W423mm × D294mm × H41mm |
|---|---|
| Materials | Aluminum, Tempered Glass, Stainless steel, Polycarbonate, others |
| Compatible bag | WELLBAG-L350 |
| Weight | 3.8kg |
Product Numbers
WELLBAGｰL Series
| Name | WELLBAGｰL350 |
|---|---|
| Size | 292mm×412mm |
| Material | Polyethylene with non-cell adhesion coating |
| Culture area | 1,000cm2 |
| Medium volume | 200～400mL |
| Well diameter | 350μm |
| Well depth | ca. 150μm |
| Number of wells | ca. 650,000 |
| Air bubble removal method | Incubation Method |
| Product number |
11020-01 (1 bag) 11020-05 (5 bags) |
WELLBAG-L Dedicated Holder
| Name | Quantity (units) | Product number |
|---|---|---|
| WELLBAG-L Dedicated Holder | 1 | 62000-01 |
Product Documents
Brochure
Reference Materials
Application
Videos
WELLBAG-L350 Operation Overview Video
FAQ
You can purchase it from our contracted distributors. However, since it is made to order, please contact us as early as possible.
It is made of a high-gas-permeability grade of polyethylene.
Single use is recommended. From the perspectives of experimental reproducibility and sterility, reuse is not recommended.
We recommend using the dedicated holder. Using the holder helps form spheroids with a more uniform diameter.
No, it is not compatible with autoclave sterilization. We recommend disinfection with alcohol, hydrogen peroxide solution, or similar agents.
No, only the 350 μm well diameter is available.
No, it cannot.
Please refer here for details.
Adjust the number of seeded cells per well according to the target spheroid diameter. Please refer here for details.
Please refer here for details.
Please refer here for details.
If there are differences in the temperature of the medium, holder, or working environment when the cells settle, thermal convection may occur inside the bag, causing non-uniformity. Therefore, we recommend setting the temperature of the medium, holder, and working environment to 37°C and allowing the cells to settle.
For WELLBAG-L350, when preparing the cell suspension, adjust it to 37°C. To remove bubbles, after taking the warmed WELLBAG with the holder out of the incubator, promptly return the holder to the incubator, quickly seed the cell suspension, remove bubbles, set it in the holder, and leave it undisturbed in the incubator to allow the cells to settle. If you have a hot plate, please use it for work inside the safety cabinet.
Three years from the date of manufacture.